Tris-HCl Buffer, pH 7.0 [1M] - 250mL
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Tris-HCl Buffer, pH 7.0 [1M] - 250mL
Manufactured in an ISO 9001 and 13485 certified facility in the USA under stringent Good Manufacturing Practices.
Tris-HCl Buffer, pH 7.0 [1M] - 250mL
Articol nr.: 97963856

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

Articol nr.: 97963856

RON 716

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Ce Iese în Evidență

Optimal pH Stability
This Tris-HCl buffer maintains a consistent pH of 7.0, ideal for biochemical assays and molecular biology applications, ensuring reliable experimental conditions and reproducible results.
High Concentration
At 1M concentration, this buffer solution offers a robust capability to resist pH changes, making it suitable for various intricate laboratory procedures involving sensitive reactions.
Convenient Volume Size
Available in a 250mL size, this buffer is perfect for regular laboratory use, minimizing waste while providing ample quantity for multiple experiments or applications.

Detalii produs

Shop Tris-HCl Buffer, pH 7.0 [1M] - 250mL online at a best price in Romania. B09V5MMXFD
Item Weight0.55 lbs (250 grams)

Cine Ar Trebui să Cumpere?

Suitable For
  • Biochemistry Students

    Ideal for laboratory experiments involving enzyme reactions and protein studies at a controlled pH level.

  • Molecular Biology Research

    Essential for applications like PCR, gel electrophoresis, and DNA/RNA manipulation requiring stable pH.

  • Clinical Labs

    Useful in diagnostic tests that require precise buffer solutions for maintaining biological sample integrity.

Not Suitable For
  • Field Research

    Not suitable for outdoor experiments due to stability issues and potential for buffer degradation over time.

  • General Household Use

    Not intended for non-scientific uses; inappropriate for cooking or home applications outside laboratory settings.

  • High pH Applications

    Inadequate for reactions or experiments needing pH levels above 7, as it specifically buffers at pH 7.0.

DESCRIEREA PRODUSULUI

Tris-HCl Buffer, pH 7.0 [1M] - 250mL

Aveți Întrebări? Discutați cu noi

Întrebări și răspunsuri ale clienților

  • întrebare: What is Tris-HCl Buffer, pH 7.0 used for?

    Răspuns: Tris-HCl Buffer, pH 7.0 is commonly used in biological and biochemical applications. It helps to maintain a stable pH environment that is essential for enzyme activity, DNA/RNA electrophoresis, and protein purification. The Tris buffer effectively minimizes pH fluctuations, allowing researchers to achieve more reproducible results in experiments. For example, in molecular biology experiments, a stable pH is crucial during reactions such as PCR or gel electrophoresis, where variations could lead to distorted or inaccurate results.
  • întrebare: How do you prepare Tris-HCl Buffer at pH 7.0?

    Răspuns: To prepare Tris-HCl Buffer at pH 7.0, you need to dissolve Tris base in distilled water and adjust the pH with hydrochloric acid (HCl) to the desired level. The buffering capacity will depend on the concentration of Tris and the final volume. For practical purposes, pre-made 1M solutions like Tris-HCl Buffer, pH 7.0, provide convenience and consistency, especially in labs where precise pH values are critical. This method is routinely applied in research facilities for applications that require consistent buffering capacity.
  • întrebare: Can Tris-HCl Buffer be used in protein assays?

    Răspuns: Yes, Tris-HCl Buffer, pH 7.0, is widely used in protein assays. Its neutral pH is compatible with many proteins, which ensures their stability during analysis. For instance, it is remarkable for techniques like Western blotting and ELISA, where protein stability and enzymatic activity are paramount. Using the buffer prevents denaturation and maintains the structural integrity of proteins throughout the assay process, making it a go-to solution for researchers working on enzyme-linked studies.
  • întrebare: Is Tris-HCl Buffer suitable for electrophoresis?

    Răspuns: Absolutely! Tris-HCl Buffer, pH 7.0, is specifically formulated for electrophoresis applications. The buffer provides optimal conditions for the migration of DNA and RNA through gel matrices during electrophoretic techniques. It also helps to maintain the stability of nucleic acids, ensuring that they do not degrade during the process. Often, labs rely on this buffer in both agarose and polyacrylamide gel electrophoresis, where a consistent pH is crucial for resolving nucleic acid fragments accurately.
  • întrebare: What volume of Tris-HCl Buffer should I use in experiments?

    Răspuns: The volume of Tris-HCl Buffer, pH 7.0, to use in experiments varies depending on the specific protocol you are following. Commonly, for reactions and assays, volumes of 50-200 µL are typically sufficient for small-scale experiments, while larger setups may require several milliliters. By referencing the specific requirements of your experiment, you can ensure you are using the appropriate buffer volume for optimal results, supporting consistent outcomes throughout various laboratory applications.
  • întrebare: How should Tris-HCl Buffer be stored?

    Răspuns: Tris-HCl Buffer, pH 7.0, should be stored at room temperature or in a cool place and kept tightly sealed to prevent contamination. It's advisable to protect it from extreme temperatures or direct sunlight. Under these conditions, the buffer can be stored for extended periods while maintaining its stability. This practice is particularly important in laboratory settings where consistent results are needed, ensuring that the buffer maintains its pH and efficacy for future experiments.
  • întrebare: Can Tris-HCl Buffer be used for DNA or RNA storage?

    Răspuns: Tris-HCl Buffer, pH 7.0, can indeed be used for storing DNA or RNA samples, as it helps to prevent degradation by providing a stable pH environment. Storing nucleic acids in this buffer allows for long-term preservation without compromising their integrity, making it ideal for sample management in molecular biology. This usage is especially beneficial in lab settings when preparing samples for further analysis or experimentation, where maintaining their quality is paramount.
  • întrebare: What are the benefits of using a pre-made Tris-HCl Buffer solution?

    Răspuns: Using a pre-made Tris-HCl Buffer, pH 7.0 streamlines laboratory processes by ensuring correct concentrations and pH values are achieved without requiring additional calculations or adjustments. This saves time and reduces the possibility of error in preparation, which is critical for experiments demanding precision. Pre-made buffers are particularly valuable in high-throughput scenarios or collaborative research, where multiple scientists rely on the same consistent buffer for their methodologies.
  • întrebare: What are the common applications of Tris-HCl Buffer in research?

    Răspuns: Tris-HCl Buffer, pH 7.0, is versatile and finds applications in various domains, such as molecular biology, biochemistry, and genetic research. Common uses include enzyme assays, protein purification, PCR, and nucleic acid gel electrophoresis. Its ability to maintain consistent pH makes it an essential reagent during experiments that demand precision, thus facilitating accurate interpretation of results and enhancing reproducibility across experiments or research laboratories.
  • întrebare: Where can I buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL?

    Răspuns: You can buy Tris-HCl Buffer, pH 7.0 [1M] - 250mL from Ubuy in Romania. Ubuy offers a convenient platform for purchasing various laboratory supplies, including specialized buffers like Tris-HCl. Their inventory ensures that you can find quality products delivered directly to your location, catering to the needs of researchers and laboratory professionals looking for reliable reagents.

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Recenziile și evaluările clienților

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Pro

  • Stable pH for experiments
  • Easy to use
  • High-quality formulation
  • Versatile for various applications
  • Trusted by researchers

Contra

  • Bottle design could be improved.

Istoric de Preț al Produsului

Important

  • Limite: Pentru produsele expediate la nivel internațional, vă rugăm să rețineți că este posibil ca garanția producătorului să nu fie valabilă; este posibil ca opțiunile de service ale producătorului să nu fie disponibile; este posibil ca manualele, instrucțiunile și avertismentele de siguranță ale produsului să nu fie în limba țării de destinație; este posibil ca produsele (și materialele însoțitoare) să nu fie proiectate în conformitate cu standardele, specificațiile și cerințele privind etichetarea din țara de destinație; este posibil ca produsele să nu fie conforme cu voltajul și cu alte standarde electrice din țara de destinație (necesitând utilizarea unui adaptor sau convertor, dacă este cazul). Destinatarul este responsabil pentru asigurarea faptului că produsul poate fi importat legal în țara de destinație. Când comandați de pe Ubuy sau de la afiliații acestuia, destinatarul este importatorul înregistrat și trebuie să respecte toate legile și reglementările din țara de destinație
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RON 716

Comandați acum și primiți Sunday, Iulie 12

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Tranzacție SecurizatăTranzacție Securizată

Ubuy depune eforturi pentru a vă proteja securitatea și confidențialitatea. Sistemul nostru avansat de securitate a plăților asigură confidențialitatea prin criptarea informațiilor dvs. în timpul transmisiei folosind protocoalele AES (Advanced Encryption Standards) și SSL (Secure Socket Layer). Detaliile dvs. de plată sunt 100% sigure, deoarece nu partajăm datele dvs. de plată cu vânzători terți.

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Cea mai rapidă livrare transfrontalieră

Caracteristici și avantaje

  • Comprehensive range of biological buffers for research and diagnostics.
  • Produced in an ISO certified facility ensuring quality.
  • Aseptically filtered for purity and reliability.
  • Suitable for protein research and molecular biology applications.
  • Complete Certificate of Analysis provided for each lot.
  • Dilutable Tris-HCl buffer for tailored concentration needs.