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Articol nr.:
84061248
Citrate Buffer, 1M, (pH 6.0), 250 mL
Articol nr.:
84061248
RON 323
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Detalii produs
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- Citrate buffers can be used for RNA isolation, due to its ability to prevent base hydrolysis. The buffer is also used for antigen detection by breaking cross-links between antigens and any substances in its fixation medium. Citrate buffer is a popular choice for immunofluorescent staining applications, as it can aid in stain intensity without contributing to background signal. It is a popular choice for biological research and clinical applications, including pathology.
| Item Form | Liquid |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 250 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.133 |
| Included Components | Bottle |
| Brand | eCon-Lab-Supply |
| Item Volume | 250 Milliliters |
What's in the box
Bottle
DESCRIEREA PRODUSULUI
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Întrebări și răspunsuri ale clienților
-
întrebare:
What is Citrate Buffer, 1M, pH 6.0 used for?
Răspuns: Citrate Buffer, 1M, pH 6.0, is primarily utilized in biochemical applications, particularly in molecular biology and biochemistry. It acts as a stabilizing agent that maintains pH conditions during various experiments, including enzyme reactions and protein assays. This buffer is essential for ensuring consistent results in DNA and RNA studies, as many assays require a specific pH for optimal performance. -
întrebare:
How do you prepare Citrate Buffer, 1M, pH 6.0?
Răspuns: To prepare Citrate Buffer, 1M, pH 6.0, mix 1M citric acid and sodium citrate at a ratio that achieves the desired pH level. Typically, this involves combining 0.1M citric acid and 0.1M sodium citrate in appropriate proportions and adjusting the pH using a pH meter. Proper preparation is crucial for obtaining accurate results in your experiments, particularly when testing acids or bases, or calibrating pH-sensitive equipment. -
întrebare:
Can I use Citrate Buffer for cell culture applications?
Răspuns: Citrate Buffer, 1M, pH 6.0 is not typically used directly in cell culture applications, but it can be beneficial for specific experimental setups that require a stable pH environment. For example, researchers might use it for washing cells or preparing reagents where pH stabilization is crucial. Always consider the specific requirements of your cell line before application, as pH sensitivity varies across different cell types. -
întrebare:
Is Citrate Buffer, 1M, pH 6.0 suitable for protein analyses?
Răspuns: Yes, Citrate Buffer, 1M, pH 6.0 is ideal for protein analyses, particularly in assays involving enzyme activity and protein stability. Many proteins function optimally at a pH around 6.0, making this buffer perfect for maintaining conditions that are conducive to accurate measurements. This buffer helps minimize fluctuations that could impact enzyme kinetics or structural integrity during analysis. -
întrebare:
What are the storage requirements for Citrate Buffer, 1M, pH 6.0?
Răspuns: Citrate Buffer, 1M, pH 6.0 should be stored in a cool, dry place, away from direct sunlight, to maintain its stability. Ideally, it should be stored at room temperature, in a tightly sealed container to prevent contamination. Proper storage is essential because it ensures the buffer remains effective for its intended applications, which may involve metabolic or chemical reactions sensitive to environmental changes. -
întrebare:
Can Citrate Buffer be used in clinical laboratories?
Răspuns: Yes, Citrate Buffer, 1M, pH 6.0 finds application in clinical laboratories, particularly in diagnostic assays where maintaining a specific pH is vital for accuracy. It is often used in coagulation studies and sample preparations where pH can affect the behavior of various biomolecules. Its reliable performance makes it a staple in routine laboratory practices. -
întrebare:
What precautions should I take when using Citrate Buffer?
Răspuns: When using Citrate Buffer, 1M, pH 6.0, it is essential to wear appropriate PPE, including gloves and goggles, to protect against potential splashes. Additionally, ensure that your workspace is clean and free from contaminants that could affect experimental results. Practicing good laboratory hygiene is critical to maintaining the integrity of your experiments and avoiding cross-contamination. -
întrebare:
Is Citrate Buffer compatible with other buffer systems?
Răspuns: Citrate Buffer, 1M, pH 6.0 can be compatible with certain other buffer systems depending on the specific requirements of your application. For example, it may be mixed with phosphate buffers in certain scenarios to achieve a desired pH or ionic strength. However, care must be taken, as combining buffers can lead to unpredictable results; it’s pivotal to test compatibility for your specific experimental conditions. -
întrebare:
What is the role of pH in experiments using Citrate Buffer?
Răspuns: In experiments involving Citrate Buffer, pH plays a crucial role in influencing molecular interactions and enzyme activity. Many biochemical processes are pH-dependent; thus, maintaining a stable pH at 6.0 is essential for achieving reproducibility in experimental results. It's particularly relevant in enzyme assays where deviations from optimal pH can lead to altered reaction rates or product formation. -
întrebare:
Where can I buy Citrate Buffer, 1M, pH 6.0, 250 mL in Romania?
Răspuns: You can purchase Citrate Buffer, 1M, pH 6.0, 250 mL from Ubuy in Romania. Ubuy provides a reliable platform for sourcing quality laboratory reagents, including this essential buffer. By shopping there, you can ensure that you are getting a product that meets high standards for your experimental and research needs.
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Pro
- Highly effective pH stabilizer
- Easy to use and measure
- Compatible with various applications
- Reliable performance across experiments
- Well-packaged for safe handling
Contra
- Occasional sediment formation
Istoric de Preț al Produsului
Important
- Limite: Pentru produsele expediate la nivel internațional, vă rugăm să rețineți că este posibil ca garanția producătorului să nu fie valabilă; este posibil ca opțiunile de service ale producătorului să nu fie disponibile; este posibil ca manualele, instrucțiunile și avertismentele de siguranță ale produsului să nu fie în limba țării de destinație; este posibil ca produsele (și materialele însoțitoare) să nu fie proiectate în conformitate cu standardele, specificațiile și cerințele privind etichetarea din țara de destinație; este posibil ca produsele să nu fie conforme cu voltajul și cu alte standarde electrice din țara de destinație (necesitând utilizarea unui adaptor sau convertor, dacă este cazul). Destinatarul este responsabil pentru asigurarea faptului că produsul poate fi importat legal în țara de destinație. Când comandați de pe Ubuy sau de la afiliații acestuia, destinatarul este importatorul înregistrat și trebuie să respecte toate legile și reglementările din țara de destinație
- Nu toate produsele listate pe Ubuy sunt de vânzare, deoarece Ubuy este un motor de căutare global. Produsele sunt supuse reglementărilor privind exportul/comerțul.
RON 323
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Tranzacție Securizată
Ubuy depune eforturi pentru a vă proteja securitatea și confidențialitatea. Sistemul nostru avansat de securitate a plăților asigură confidențialitatea prin criptarea informațiilor dvs. în timpul transmisiei folosind protocoalele AES (Advanced Encryption Standards) și SSL (Secure Socket Layer). Detaliile dvs. de plată sunt 100% sigure, deoarece nu partajăm datele dvs. de plată cu vânzători terți.
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Caracteristici și avantaje
- Comprehensive range of biological buffers
- Buffers important for protein research and molecular biology
- Manufactured under stringent Good Manufacturing Practices
- Citrate buffer's versatile applications in RNA isolation and immunofluorescent staining
- Popular choice for biological research and clinical applications
- For research use only. Not for human or animal clinical/therapeutic use