20x Tris-Acetate-SDS Running Buffer (500 ML)
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20x Tris-Acetate-SDS Running Buffer (500 ML)
Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer.
20x Tris-Acetate-SDS Running Buffer (500 ML)
Articol nr.: 125660636

20x Tris-Acetate-SDS Running Buffer (500 ML)

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Articol nr.: 125660636

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Tris-Acetate gels provide excellent separation of large molecular weight proteins when used with Tris-Acetate SDS Running Buffer.
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Ce Iese în evidență

High Purity
This running buffer is formulated with high-purity reagents, ensuring reliable and reproducible results in electrophoresis applications, making it ideal for both research laboratories and educational institutions.
Convenience Pack
The convenient 500 ML packaging allows for easy handling and storage, providing sufficient volume for multiple experiments while maintaining product stability and prolonging shelf life.
Versatile Application
Designed for Tris-Acetate-SDS systems, this running buffer supports a range of protein separation techniques, catering to diverse scientific needs and enhancing productivity in both small-scale and large-scale experiments.

Detalii produs

Shop 20x Tris-Acetate-SDS Running Buffer (500 ML) online at a best price in România. B0DJDRVT3L
Brand NameGeneric
Model NumberTAS01-500ML
Number of Items1
ManufacturerBioland Scientific LLC
Manufacturer Part NumberTAS01-500ML
BrandGeneric
Item Weight1.1 lbs (500 grams)

Cine Ar Trebui să Cumpere?

Suitable For
  • Molecular Biologists

    Ideal for molecular biologists conducting SDS-PAGE experiments to analyze protein samples effectively.

  • Educational Institutions

    Well-suited for labs and classrooms focusing on protein separation techniques in educational settings.

  • Protein Research

    Beneficial for researchers studying protein interactions, structures, and molecular weight using electrophoresis.

Not Suitable For
  • Casual Hobbyists

    Not suitable for casual users without proper lab equipment and knowledge of protein analysis.

DESCRIEREA PRODUSULUI

20x Tris-Acetate-SDS Running Buffer (500 ML)

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Întrebări și răspunsuri ale clienților

  • Întrebare: What is 20x Tris-Acetate-SDS Running Buffer used for?

    Răspuns: 20x Tris-Acetate-SDS Running Buffer is primarily used for protein electrophoresis in molecular biology labs. It facilitates the separation of proteins based on their size during gel electrophoresis. The buffer's high ionic strength and unique composition help stabilize pH levels during the run, ensuring more precise and reproducible results. Researchers often utilize this buffer when analyzing protein samples, confirming protein purity, or assessing molecular weight for better understanding of protein behavior in processes like Western blotting.
  • Întrebare: How do you prepare the 1x working solution from 20x Tris-Acetate-SDS Running Buffer?

    Răspuns: To prepare a 1x working solution from a 20x Tris-Acetate-SDS Running Buffer, simply dilute 1 part of the 20x buffer with 19 parts of distilled water. For example, if you need 500 mL of 1x solution, mix 25 mL of the 20x buffer with 475 mL of distilled water. This simple dilution effectively adjusts the buffer concentration for optimal performance in gel electrophoresis, helping to ensure precise protein separation and enhanced analysis.
  • Întrebare: What types of samples can be run using this buffer?

    Răspuns: This buffer is ideal for a variety of protein samples such as purified proteins, cell lysates, or recombinant proteins. It is commonly used for analyzing proteins extracted from biological tissues, microorganisms, or cultured cells. By ensuring consistent pH stability and optimal ionic strength, it enables accurate size-based separation of various protein types during SDS-PAGE. This versatility makes it a staple in laboratories focusing on protein research, diagnostics, or biotechnology applications.
  • Întrebare: Is 20x Tris-Acetate-SDS Running Buffer suitable for Western blotting?

    Răspuns: Yes, 20x Tris-Acetate-SDS Running Buffer is suitable for Western blotting applications. After proteins are separated by SDS-PAGE using this buffer, they can be transferred to a membrane for further analysis. The buffer's composition promotes effective protein migration and maintaining structural integrity, which is crucial for accurate detection later in the Western blotting process. Many researchers depend on it for reliable results in protein identification and quantification.
  • Întrebare: What storage conditions are recommended for this buffer?

    Răspuns: 20x Tris-Acetate-SDS Running Buffer should be stored at room temperature, away from direct sunlight and extreme temperature fluctuations. Proper storage helps maintain its chemical stability and effectiveness for electrophoresis. Additionally, always ensure that the bottle is tightly sealed to prevent contamination or evaporation of essential components. Adequate storage practices extend the buffer’s usability, making it ready for your experiments whenever needed.
  • Întrebare: What is the pH range of the 1x working solution?

    Răspuns: The pH of the 1x working solution of 20x Tris-Acetate-SDS Running Buffer typically ranges between 7.2 and 8.4, depending on the specific formulation. This pH range is critical for maintaining the integrity of proteins during electrophoresis while ensuring optimal separation conditions. Many users find that this pH range supports a wide array of protein types, enhancing the reproducibility of their experimental outcomes.
  • Întrebare: Can this buffer be used for DNA electrophoresis as well?

    Răspuns: No, 20x Tris-Acetate-SDS Running Buffer is specifically designed for protein electrophoresis. The presence of SDS in the buffer denatures protein structures, allowing them to be separated based on size. For DNA electrophoresis, a different buffer, such as TAE or TBE, is typically used as they do not denature nucleic acids and provide appropriate charge and stability for DNA fragments. It’s essential to choose the right buffer depending on your analysis needs.
  • Întrebare: What equipment do I need to use this running buffer?

    Răspuns: To utilize 20x Tris-Acetate-SDS Running Buffer, you'll need basic equipment such as an electrophoresis unit, power supply, and gel casting trays. Additionally, gel electrophoresis apparatus like combs and measuring devices are advisable for precise setup. This equipment works in tandem to ensure an effective separation process. Proper preparation and setup using the right tools enhance the efficiency and accuracy of your protein electrophoresis experiments.
  • Întrebare: What are the safety precautions when using this buffer?

    Răspuns: When using 20x Tris-Acetate-SDS Running Buffer, it's important to wear appropriate personal protective equipment (PPE) such as gloves, lab coats, and safety goggles. The buffer contains SDS, which can be irritating to the skin and eyes. Therefore, avoid direct contact, and always work in a well-ventilated area. Additionally, ensure proper lab protocols are followed for disposal of any waste or spillage to maintain a safe working environment.
  • Întrebare: Where can I buy 20x Tris-Acetate-SDS Running Buffer 500 ML in Romania?

    Răspuns: You can buy 20x Tris-Acetate-SDS Running Buffer 500 ML on Ubuy. Ubuy offers a wide selection of laboratory supplies, including running buffers, enabling you to find the right products for your scientific needs. Their platform also provides important details about the product specifications and usage to assist you in making an informed purchase.

Buffers TAS01-500ML Editorial Review

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Caracteristici și avantaje

  • Highly effective for protein separation in denatured states.
  • Optimized for large molecular weight proteins.
  • Can be used with both Tris-Acetate SDS and LDS Sample Buffers.
  • Compatible with Tris-Glycine Native Running Buffer for more effective native protein resolution.
  • Stable for one year when stored at 4°C.
  • Enhances the performance of Tris-Acetate gels significantly.

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