PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit
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PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit
Apex Liquid Buffers are crafted using ultra pure or cGMP grade chemicals, ensuring high quality for all your molecular biology needs.
PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit
Articol nr.: 181073531

PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit

Articol nr.: 181073531

RON 636

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Ce Iese în Evidență

Concentrated Solution
This 10X concentrated PBS-Tween 20 solution saves storage space and minimizes shipping costs, providing an efficient and economical option for laboratories requiring high-quality reagents.
Ideal pH Balance
With a precise pH of 7.4, this solution mimics physiological conditions, ensuring optimal performance in various biological applications, enhancing reproducibility in your experiments.
Bulk Quantity
The 500ml unit size is perfect for research labs, allowing for prolonged use without frequent reorders, ensuring uninterrupted research workflows and productivity.

Detalii produs

Shop PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit online at a best price in Romania. B07Q3HMTHK
Container TypeBottle
Item FormLiquid
Volume1000 Milliliters
Brand NameAPEX
ManufacturerApex Bioresearch Products
Part Number18-174
BrandAPEX
Item Volume1000 Milliliters
Package InformationBottle
Item Weight1.1 lbs (500 grams)

Cine Ar Trebui să Cumpere?

Suitable For
  • Laboratory Researchers

    Ideal for researchers needing a reliable buffer solution for biological experiments and assays requiring precise pH conditions.

  • Diagnostic Facilities

    Perfect for assay development and standard testing, providing consistency for numerous diagnostic procedures in clinical settings.

  • Biotechnology Companies

    Useful for companies conducting enzyme assays, Western blots, and immunoassays, ensuring accurate experimental conditions.

Not Suitable For
  • Home Users

    Not suitable for general home use or non-scientific applications, as it's specifically designed for laboratory settings.

DESCRIEREA PRODUSULUI

PBST (PBS-Tween 20), 10X, pH 7.4, 1000ml/Unit

Aveți Întrebări? Discutați cu noi

Întrebări și răspunsuri ale clienților

  • întrebare: What is PBST (PBS-Tween 20) used for?

    Răspuns: PBST, or PBS-Tween 20, is commonly used in biological and biochemical research. It acts as a buffer solution that maintains a stable pH and is particularly effective in wash steps during cell and protein assays. The addition of Tween 20, a non-ionic detergent, reduces non-specific binding of proteins to surfaces, improving the clarity and accuracy of experimental results. This makes PBST particularly valuable in applications like ELISA, Western blotting, and immunohistochemistry.
  • întrebare: What are the components of PBST?

    Răspuns: PBST consists of phosphate-buffered saline (PBS) and Tween 20. PBS is an isotonic buffer containing sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate, which helps maintain osmotic balance. Tween 20 is a surfactant that helps reduce protein aggregation and facilitates better penetration in assays, leading to more reliable outcomes. Together, these components optimize conditions for various biological applications.
  • întrebare: How do you prepare PBST from the 10X stock solution?

    Răspuns: To prepare PBST from the 10X stock solution, simply dilute the stock solution in distilled water to reach a final concentration. For example, mix one part of the 10X PBST with nine parts of distilled water to achieve a 1X solution. This dilution ensures that the pH and buffer capacity of the solution are maintained, allowing for optimal performance in your assays. Always check the pH as a best practice to ensure it remains around 7.4.
  • întrebare: What is the significance of the pH 7.4 in PBST?

    Răspuns: The pH of 7.4 in PBST is critical as it closely matches the physiological pH of most biological systems. This neutral pH ensures that proteins and cells remain stable and functional during experimental processes. Maintaining this pH level can greatly influence the efficiency of antibody binding and protein interactions, making it vital in experiments such as immunoassays where biological relevance is key.
  • întrebare: Can PBST be stored for later use?

    Răspuns: Yes, PBST can be stored for future use, provided it is kept under appropriate conditions. Store the solution at 4°C to maintain its stability and effectiveness. However, it’s advisable to avoid repeated freeze-thaw cycles which can degrade the solution's components. When stored correctly, PBST can remain effective for several weeks, making it convenient for multiple experiments.
  • întrebare: Is PBST safe to use in a laboratory environment?

    Răspuns: PBST is generally considered safe for use in laboratory environments, with no known toxic effects from its components. However, like any reagent, it should be handled with care, using appropriate personal protective equipment, such as gloves and goggles. It is always good practice to review the safety data sheet (SDS) for proper handling guidelines to ensure a safe working environment.
  • întrebare: What are some common applications of PBST in research?

    Răspuns: PBST is widely used in various research applications, including ELISA assays for quantitative analysis of proteins and Western blotting for the detection of specific proteins. Moreover, it is beneficial in immunocytochemistry techniques to detect antigens in cells and tissues. Its role in these applications enhances the reliability and clarity of results, crucial for scientific studies.
  • întrebare: How does the presence of Tween 20 affect experimental outcomes?

    Răspuns: The inclusion of Tween 20 in PBST enhances experimental outcomes by minimizing non-specific binding and improving the accessibility of antibodies to their targets. This surfactant disperses proteins and helps maintain their stability, resulting in clearer assay results. Consequently, using PBST with Tween 20 can significantly enhance sensitivity and specificity in applications such as ELISA and Westerns.
  • întrebare: What is the shelf-life of PBST once opened?

    Răspuns: Once opened, PBST should ideally be used within a certain timeframe to ensure its effectiveness, typically within 2-4 weeks when stored at 4°C. The shelf-life can be affected by factors such as contamination or exposure to light. To maximize its usability, it is recommended to avoid touching the inside of the cap or the opening of the bottle, ensuring the integrity of the solution.
  • întrebare: Where can I buy PBST (PBS-Tween 20), 10X, pH 7.4, 500ml/Unit in Romania?

    Răspuns: You can purchase PBST (PBS-Tween 20), 10X, pH 7.4, 500ml/Unit from Ubuy in Romania. Ubuy offers a variety of laboratory reagents and supplies with the convenience of online shopping, ensuring you can easily find and acquire the essential products you need for your research.

APEX Buffers Editorial Review

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RON 636

Comandați acum și primiți Miercuri, August 19

QTY:

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Caracteristici și avantaje

  • Uses ultra pure or cGMP grade chemicals for maximum quality.
  • Contains 4 fold purified water for enhanced performance.
  • Ideal for molecular biology research and protein analysis.
  • Purity exceeds 99%, ensuring reliable results.
  • Free from RNase, DNase, endonucleases, endotoxins, proteins, and proteases.
  • Perfect for purification, crystallization, and diagnostic applications.

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